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KMID : 0811720100140030163
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 3 p.163 ~ p.167
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Morinda citrifolia Inhibits Both Cytosolic Ca2＋-dependent Phospholipase A2 and Secretory Ca2＋-dependent Phospholipase A2
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Song Ho-Sun
Jeong Jae-Min
Ko Myoung-Soo
PArk Sung-Hun
Sim Sang-Soo
Son Uy-Dong
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Abstract
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This study investigated the effects of the methanol extracts of Morinda citrifolia containing numerous anthraquinone and iridoid on phospholipase A2 (PLA2) isozyme. PLA2 activity was measured using various PLA2 substrates, including 10-pyrene phosphatidylcholine, 1-palmitoyl-2-[14C]arachidonyl phosphatidylcholine ([14C]AA-PC), and [3H]arachidonic acid (AA). The methanol extracts suppressed melittin-induced [3H]AA release in a concentration-dependent manner in RAW 264.7 cells, and inhibited cPLA2/sPLA2-induced hydrolysis of [14C]AA-PC in a concentration- and time-dependent manner. A Dixon plot showed that the inhibition by methanol extracts on cPLA2 and sPLA2 appeared to be competitive with inhibition constants (Ki) of 3.7?g/ml and 12.6?g/ml, respectively. These data suggest that methanol extracts of Morinda citrifolia inhibits both Ca2＋-dependent PLA2 such as, cPLA2 and sPLA2. Therefore, Morinda citrifolia may possess anti-inflammatory activity secondary to Ca2＋- dependent PLA2 inhibition.
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KEYWORD
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Morinda citrifolia, Phospholipase A2, Arachidonic acid
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